测试ID: HCCDD
尸体或溶血标本，有症状的血清中的丙型肝炎病毒抗体

ORTHO HCV Version 3.0 ELISA检测系统是在一个被重组丙型肝炎病毒(HCV)抗原(c22-3, c200和NS5)组合包被的微孔中进行的3阶段测试。在第一阶段，稀释的试验标本或适当的对照在试验井中孵育一段指定的时间。如果标本中存在对3种抗原反应的抗体，则在微孔表面形成抗原-抗体复合物。如果抗- hcv不存在，复合物就不会形成。在随后的清洗步骤中，未结合的血清蛋白将被去除。在第二阶段，将偶联到辣根过氧化物酶的小鼠单克隆抗体添加到微孔中。该偶联物特异性地结合到抗原-抗体复合物的人IgG部分。如果抗原-抗体复合物不存在，未结合的结合物将被随后的洗涤去除。第三阶段，在测试井中加入邻苯二胺(OPD)和过氧化氢组成的酶检测系统。如果有结合的共轭物存在，则OPD将被氧化，产生有颜色的最终产物。 In this reaction, peroxidase is divalently oxidized by hydrogen peroxide to form an intermediate compound, which is, in turn, reduced to its initial state by subsequent interaction with hydrogen ion donating OPD. The resulting oxidized form of OPD has an orange color. Sulfuric acid is then added to stop the reaction. The color intensity is dependent upon the amount of bound conjugate and is a function of the concentration of anti-HCV present in the specimen. The color intensity is measured with a microwell reader (photometer) designed to measure light absorbance in a microwell (Package insert: Hepatitis C Virus Encoded Antigen [Recombinant c22-3, c200, and NS5], ORTHO HCV Version 3.0 ELISA Test System. Ortho Clinical Diagnostics; 01/2017)

星期一,星期四