| web: | Mayocliniclabs.com |
|---|---|
| email: | mcl@mayo.edu |
| telephone: | 800-533-1710 |
| 国际的： | +1 855-379-3115 |
| 值仅在打印当天有效 | |
test Id :pbl我
plasma Cell Assessment, Blood
overview
对...有用
suggests clinical disorders or settings where the test may be helpful
suggests clinical disorders or settings where the test may be helpful
detecting peripheral blood involvement by plasma cell proliferative disorders
establishing the diagnosis of and determining prognosis for plasma cell proliferative disorders
测试算法
delineates situations when tests are added to the initial order. This includes reflex and additional tests.
delineates situations when tests are added to the initial order. This includes reflex and additional tests.
special Instructions
library of PDFs including pertinent information and forms related to the test
library of PDFs including pertinent information and forms related to the test
method Name
Ashort description of the method used to perform the test
Ashort description of the method used to perform the test
Flow Cytometry
nystate Available
我ndicates the status of NY State approval and if the test is orderable for NY State clients.
我ndicates the status of NY State approval and if the test is orderable for NY State clients.
yes
报告名称
列出了测试的已发表名称的简短或缩写版本
列出了测试的已发表名称的简短或缩写版本
plasma Cell Assessment, B
别名
lists additional common names for a test, as an aid in searching
lists additional common names for a test, as an aid in searching
外周血标签指数
测试算法
delineates situations when tests are added to the initial order. This includes reflex and additional tests.
delineates situations when tests are added to the initial order. This includes reflex and additional tests.
specimen Type
描述了测试验证的样品类型
描述了测试验证的样品类型
whole blood
shipping Instructions
标本必须在收集的72小时内到达。
necessary Information
date and time of collection是必要的。
specimen Required
defines the optimal specimen required to perform the test and the preferred volume to complete testing
defines the optimal specimen required to perform the test and the preferred volume to complete testing
Container/Tube:
首选：Green top (sodium heparin)
Acceptable:lavender top (EDTA)
specimen Volume:10 mL
收集说明：
1.不要离心。
2. Send whole blood specimen in original tube.do not aliquot.
special Instructions
library of PDFs including pertinent information and forms related to the test
library of PDFs including pertinent information and forms related to the test
形式
我f not ordering electronically,complete, print, and send a血液病理/细胞遗传学测试请求(T726) with the specimen.
specimen Minimum Volume
定义由测试实验室确定的提供临床相关结果所需的样本数量
定义由测试实验室确定的提供临床相关结果所需的样本数量
4 mL
拒绝due To
标识可能导致样品被拒绝的样本类型和条件
标识可能导致样品被拒绝的样本类型和条件
| 严重的溶血 | 拒绝 |
specimen Stability Information
provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| specimen Type | temperature | time | 特殊容器 |
|---|---|---|---|
| whole blood | Ambient (preferred) | 72 hours | |
| refrigerated | 72 hours |
对...有用
suggests clinical disorders or settings where the test may be helpful
suggests clinical disorders or settings where the test may be helpful
detecting peripheral blood involvement by plasma cell proliferative disorders
establishing the diagnosis of and determining prognosis for plasma cell proliferative disorders
测试算法
delineates situations when tests are added to the initial order. This includes reflex and additional tests.
delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Clinical Information
discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
等离子体细胞增殖疾病是一组血液学肿瘤，所有这些都是源自克隆血浆细胞的。这些疾病表现出广泛的生物学活性，范围从具有不确定意义的单克隆性胶质病，这种疾病进展率较低，多发性骨髓瘤通常是一种疾病，这种疾病通常是侵略性的，长期生存率较差。检测血浆细胞免疫球蛋白轻链限制（即，主要的kappa或主要lambda光链的存在）是评估血浆细胞克隆性的重要因素，因此建立了诊断。此外，这些疾病的周围血液参与更大程度与更具侵略性的疾病类型有关，因此是一种不利的预后指标。
Flow cytometric immunophenotyping (FCIP) is a recognized method for detecting plasma cell immunoglobulin light chain restriction. However, short comings of the traditionally performed technique include relative insensitivity and consistent underestimation of the number of clonal plasma cells present. Both short comings are likely attributable to limitations of the instruments and antibodies used, as well as the presence of intraclonal phenotypic heterogeneity, which creates difficulties in accurately detecting and enumerating all of the clonal plasma cells. For this reason, the FCIP plasma cell clonality assessment previously performed in our laboratory was supplemented with a slide-based immunofluorescence technique.
However, recent advances in flow cytometry have led to the development of more powerful instruments and antibody reagents that allow for the use of greater antibody combinations and increased resolution of the data. With these tools, the ability of FCIP to detect and enumerate plasma cell clones has been greatly enhanced, allowing us to discontinue the supplemental, labor-intensive, slide-based plasma cell evaluation in peripheral blood specimens.
the following algorithms are available:
reference Values
describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
将提供解释性报告。
解释
provides information to assist in interpretation of the test results
provides information to assist in interpretation of the test results
我n normal peripheral blood specimens, no clonal plasma cells are present (polytypic or too few to detect).
plasma cells are CD38 and CD138 positive.
normal (polyclonal, nonneoplastic) plasma cells are typically CD19-positive, whereas neoplastic (clonal) plasma cells typically are CD19-negative. CD19 expression is especially helpful in distinguishing clonal from nonclonal plasma cells when few analyzable cells are present.
Cd45 may be expressed by both normal and neoplastic plasma cells. In some plasma cell proliferative disorders there are both CD45-positive and CD45-negative subsets within the clonal cell population.
这些抗原的评估有助于鉴定异常的浆细胞，但是，它们不会独立报告。
Cautions
讨论可能导致诊断混乱的条件，包括标本收集和处理不当，不适当的测试选择以及干扰物质
讨论可能导致诊断混乱的条件，包括标本收集和处理不当，不适当的测试选择以及干扰物质
没有明显的警告陈述
Clinical Reference
recommendations for in-depth reading of a clinical nature
recommendations for in-depth reading of a clinical nature
Chakraborty R, Muchtar E, Kumar SK, et al: Risk stratification in myeloma by detection of circulating plasma cells prior to autologous stem cell transplantation in the novel agent era. Blood Cancer J. 2016 Dec;6(12):e512. doi: 10.1038/ bcj.2016.117
serial measurements of circulating plasma cells before and after induction therapy have an independent prognostic impact in patients with multiple myeloma undergoing upfront autologous transplantation.血液学。2017年8月; 102（8）：1439-1445
3。Evans LA，Jevremovic D，Nandakumar B等人：利用多参数流式细胞仪的诊断，原发性浆细胞白血病患者。Am J Hematol。2020 Jun; 95（6）：637-642。doi：10.1002/ajh.25773
4. Gonsalves WI, Jevremovic D, Nandakumar B, et al: Enhancing the R-ISS classification of newly diagnosed multiple myeloma by quantifying circulating clonal plasma cells. Am J Hematol. 2020 Mar;95(3):310-315. doi: 10.1002/ajh.25709
5. Ravi P, Kumar SK, Roeker L, et al. Revised diagnostic criteria for plasma cell leukemia: results of a Mayo Clinic study with comparison of outcomes to multiple myeloma. Blood Cancer J. 2018 Nov;8(12):116
special Instructions
library of PDFs including pertinent information and forms related to the test
library of PDFs including pertinent information and forms related to the test
方法描述
describes how the test is performed and provides a method-specific reference
describes how the test is performed and provides a method-specific reference
血浆细胞免疫球蛋白轻链限制评估是通过6色流式细胞仪使用单个测定管进行的，该试管含有抗Kappa IG轻链，Lambda IG轻链，CD19，CD19，CD38，CD45和CD138的抗体。CD38和CD138用于在等离子体细胞上栅极，抗Kappa和抗Lambda用于鉴定细胞质IG光链。流式细胞仪筛查将报告具有免疫球蛋白轻链限制（克隆性）的可检测到的浆细胞群体。（未发表的蛋黄酱方法）
pdFreport
指示该报告是否包含带有图表，图像或其他丰富信息的其他文档
指示该报告是否包含带有图表，图像或其他丰富信息的其他文档
no
day(s) Performed
概述了进行测试的日子。该领域反映了样品必须在测试实验室中才能开始测试过程的一天，并包括进行测试之前的任何标本准备和处理时间。一些测试被列为连续执行的，这意味着测定在白天多次进行。
概述了进行测试的日子。该领域反映了样品必须在测试实验室中才能开始测试过程的一天，并包括进行测试之前的任何标本准备和处理时间。一些测试被列为连续执行的，这意味着测定在白天多次进行。
specimens processed: Monday through Sunday
结果报告：星期一至星期五
report Available
the interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
the interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
1 to 2 days
specimen Retention Time
outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
没有保留
performing Laboratory Location
指示执行测试的实验室的位置
指示执行测试的实验室的位置
罗切斯特
Fees
several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in totest Prices有关详细的费用信息。
- Clients without access to Test Prices can contact必威平台官网24 hours a day, seven days a week.
- prospective clients should contact their Regional Manager. For assistance, contact必威平台官网。
test Classification
provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
this test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
CptCode Information
为确定每个测试或配置文件的适当当前程序术语（CPT）代码提供指导。列出的CPT代码反映了梅奥诊所实验室的CPT编码要求解释。betway中国版每个实验室的责任是确定用于计费的正确CPT代码。
CPT代码由表演实验室提供。
为确定每个测试或配置文件的适当当前程序术语（CPT）代码提供指导。列出的CPT代码反映了梅奥诊所实验室的CPT编码要求解释。betway中国版每个实验室的责任是确定用于计费的正确CPT代码。
CPT代码由表演实验室提供。
CPT代码由表演实验室提供。
88184-Flow细胞术，细胞表面，细胞质
88185 x 5-Each additional marker
88187-FLOW细胞仪，解释；2到8个标记
lo我nC® Information
provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| test Id | 测试订单名称 | order LOINC Value |
|---|---|---|
| pbl我 | plasma Cell Assessment, B | 86900-8 |
| result Id | test Result Name | result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| 30388 | 血浆细胞轻链 | 86900-8 |
| 26838 | ＃单型PC每150,000个活动 | 19099-1 |
| 26839 | pCevent Interpretation | 69052-9 |
测试设置资源
setup Files
test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.
test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.
样本报告
normal and Abnormal sample reports are provided as references for report appearance.
normal and Abnormal sample reports are provided as references for report appearance.
SI样本报告
我nternational System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.
我nternational System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.
test Update Resources
| Change Type | effective Date |
|---|---|
| File Definition - Result ID | 2021-10-26 |